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Scientia Silvae Sinicae ›› 2011, Vol. 47 ›› Issue (1): 68-72.doi: 10.11707/j.1001-7488. 20110111

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Immunocytochemical Identification of Vegetative Storage Proteins in the Branch of Ginkgo biloba

Guo Hongyan1,2, Peng Fangren2, Wu Qingxia2, Bai Jinhua1   

  1. 1. College of Forestry, Shanxi Agricultural University Taigu 030801;2. College of Forest Resources and Environment, Nanjing Forestry University Nanjing 210037
  • Received:2009-11-26 Revised:2010-01-05 Online:2011-01-25 Published:2011-01-25

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Abstract:

Both 32 ku and 36 ku proteins had been in speculated as the vegetative storage proteins the branches of Ginkgo biloba based on the obvious seasonal changes in their contents. The cytochemical localization of the two proteins was studied with the indirect immuohistochemistry localization technigue. The proteins with a certain immune correlation, were distributed in the vacuole of the parenchyma cells of cortex and the parenchyma cells of phloem. The results revealed tissue distribution pattern of the two kinds of protein, and in the meantime the results provided strong evidence to support the two kinds of protein as the vegetative storage proteins of G. biloba.

Key words: Ginkgo biloba, vegetative storage proteins(VSPs), immunocytochemical localization

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